Detection of exoerythrocytic stages of Plasmodium berghei in fixed liver tissue and cultured cells by an immunoperoxidase antibody technique
Identifieur interne : 003705 ( Main/Exploration ); précédent : 003704; suivant : 003706Detection of exoerythrocytic stages of Plasmodium berghei in fixed liver tissue and cultured cells by an immunoperoxidase antibody technique
Auteurs : M. R. Hollingdale [États-Unis] ; P. Leland [États-Unis]Source :
- Transactions of the Royal Society of Tropical Medicine and Hygiene [ 0035-9203 ] ; 1982.
English descriptors
- Teeft :
- Berghei, Cell biology, Cultured cells, Electron microscopy, Exoerythrocytic, Exoerythrocytic schizonts, Exoerythrocytic stages, Hollingdale, Immunoperoxidase, Immunoperoxidase antibody reaction, Immunoperoxidase technique, Inoculation, Krotoski, Liver sections, Liver tissue, Malaria parasites, Merozoite, Normal mouse serum, Parasite, Perinuclear staining, Plasmodium, Plasmodium berghei, Research institute, Serological detection, Sporozoite, Tissue antigens, Tropical medicine.
Abstract
Abstract: An immunoperoxidase antibody (IPA) technique is described for the detection of exoerythrocytic (EE) stages of Plasmodium berghei in rat liver sections or cultured WI38 cells. EE stages were detected in liver sections 12 hours after inoculation of sporozoites, but as early as three hours after the inoculation of WI38 cells. By 42 hours in liver sections and 48 hours in WI38 cells, EE parasites were easily visible by low power microscopy, and merozoites were clearly seen by 72 hours within EE parasites in WI38 cells, or extracellularly on adjacent cells.
Url:
DOI: 10.1016/0035-9203(82)90225-5
Affiliations:
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R." last="Hollingdale">M. R. Hollingdale</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biomedical Research Institute, 12111 Parklawn Drive, Rockville, Maryland 20852</wicri:regionArea><wicri:noRegion>Maryland 20852</wicri:noRegion></affiliation></author><author><name sortKey="Leland, P" sort="Leland, P" uniqKey="Leland P" first="P." last="Leland">P. Leland</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biomedical Research Institute, 12111 Parklawn Drive, Rockville, Maryland 20852</wicri:regionArea><wicri:noRegion>Maryland 20852</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Transactions of the Royal Society of Tropical Medicine and Hygiene</title><title level="j" type="abbrev">TRSTMH</title><idno type="ISSN">0035-9203</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1982">1982</date><biblScope unit="volume">76</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="624">624</biblScope><biblScope unit="page" to="626">626</biblScope></imprint><idno type="ISSN">0035-9203</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0035-9203</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Berghei</term><term>Cell biology</term><term>Cultured cells</term><term>Electron microscopy</term><term>Exoerythrocytic</term><term>Exoerythrocytic schizonts</term><term>Exoerythrocytic stages</term><term>Hollingdale</term><term>Immunoperoxidase</term><term>Immunoperoxidase antibody reaction</term><term>Immunoperoxidase technique</term><term>Inoculation</term><term>Krotoski</term><term>Liver sections</term><term>Liver tissue</term><term>Malaria parasites</term><term>Merozoite</term><term>Normal mouse serum</term><term>Parasite</term><term>Perinuclear staining</term><term>Plasmodium</term><term>Plasmodium berghei</term><term>Research institute</term><term>Serological detection</term><term>Sporozoite</term><term>Tissue antigens</term><term>Tropical medicine</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: An immunoperoxidase antibody (IPA) technique is described for the detection of exoerythrocytic (EE) stages of Plasmodium berghei in rat liver sections or cultured WI38 cells. EE stages were detected in liver sections 12 hours after inoculation of sporozoites, but as early as three hours after the inoculation of WI38 cells. By 42 hours in liver sections and 48 hours in WI38 cells, EE parasites were easily visible by low power microscopy, and merozoites were clearly seen by 72 hours within EE parasites in WI38 cells, or extracellularly on adjacent cells.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Hollingdale, M R" sort="Hollingdale, M R" uniqKey="Hollingdale M" first="M. R." last="Hollingdale">M. R. Hollingdale</name></noRegion><name sortKey="Leland, P" sort="Leland, P" uniqKey="Leland P" first="P." last="Leland">P. Leland</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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